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Standard ratios: 2mg peptide + 2ml water = 1mg/ml (1000mcg/ml) 5mg peptide + 2ml water = 2.5mg/ml (2500mcg/ml) 5mg peptide + 1ml water = 5mg/ml (5000mcg/ml) 10mg peptide + 2ml water = 5mg/ml (5000mcg/ml) How to choose: More water (2-3ml per vial): Easier to measure small doses accurately Larger injection volumes (0.2-0.3ml) Lasts longer (more injections per vial) Less water (1ml per vial): Higher concentration Smaller injection volumes (0.05-0.1ml) Fewer injections per vial Most people use 2ml of water for 5mg vials

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Abbreviations GLP-1RAs: Glucagon-like peptide-1 receptor agonists T2DM: Type 2 diabetes mellitus DPP-4: Dipeptidyl peptidase IV GLP-1R: Glucagon-like peptide-1 receptor cAMP: Cyclic adenosine monophosphate PKA: Protein kinase A PI3K: Phosphatidylinositol 3-kinase Akt: Protein kinase B AD: Alzheimers diseases TRPV1: Transient receptor potential vanilloid 1 OA: Osteoarthritis KOA: Knee OA MIA: Monoiodoacetate IL-6: Interleukin 6 PGE2: Prostaglandin E2 NO: Nitric oxide ER: Endoplasmic reticulum IL-1: Interleukin-1 MMP-3: Matrix metalloproteinases-3 MMP-13: Matrix metalloproteinases-13 ROS: Reactive oxygen species BMI: Body mass index IBS: Irritable bowel syndrome ATP: Adenosine triphosphate SERT: Serotonin transporter TPH-1: Tryptophan hydroxylase-1 AC: Adenylate cyclase LPS: Lipopolysaccharide WAS: Water avoidance stress IBS-C: Constipation-predominant IBS SNL: Spinal nerve ligation SCI: Spinal cord injury CHOP: C/EBP homologous transcription factor protein GRP78: Glucose regulatory protein 78 PSNT: Partial sciatic nerve transection L