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described in research write-ups, not clinical trials
Before the experiment, the cells were loaded with 0.2% Fluo-4 AM, which was dissolved in HEPES-buffered Hanks Balanced Salt Solution (HBSS, Thermofisher) containing 1 mM CaCl2, 1 mM MgCl2, and 0.5% probenecid (Thermofisher) for one hour
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Identification of enteroendocrine regulators by real-time single-cell differentiation mapping
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