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Significant main effects of treatment group (prior to BenjaminiHochberg correction since none were significant after correction, although the necessity of correction for multiple comparisons in exploratory analyses remains unresolved 49 ) were observed for the unclassified group and ten classified genera, namely, Acetatifactor , Akkermansia , Erysipelatoclostridium , [ Eubacterium ] coprostanoligenes group , Family XIII UCG-001 , Lactobacillus , Parasutterella , Ruminiclostridium 6 , Ruminococcaceae UCG-004 , and Ruminococcaceae UCG-009
Sphere formation: DKK3 knockout LX2 cells or control LX2 cells co-cultured with either hepatoma cell line, Hep3b, or cholangiocarcinoma cell line, Hucct-1, in ultra-low attachment plate to form spheres
64 NioiP.NguyenT
Conclusion: Heavy drinking alone is sufficient to decrease plasma albumin, indicating that albumin decreases are an early event in ALD progression
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