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Oncogenic mutations in Ras lead to enhanced ROS formation from NADPH oxidase 4 (NOX4) to increase proliferation

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To increase bacterial density of BW25113_WT, crp or cysE bacteria, 12.5 L of overnight bacterial cultures (1x) or different concentrated suspensions of those cultures (10x, 20x, or 40x) were seeded onto NGM agar and allowed to grow for two days as indicated before behavioral tests