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After washing in PBS, primary antibodies (IgM-PE BioLegend 406507, IgD-APC BioLegend 405713, MARCO Santa Cruz sc-65353/ anti-rat PE Abcam ab97058 and Siglec-1 Biolegend 142417) diluted in PBS 10% FCS were incubated overnight at 4 C
A 2013 study by Prussick, Prussick, and Gutman outlines this further
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GHK-Cu operates by coordinating copper (II) ions and delivering them into cells, where they act as cofactors for vital enzymes
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