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For MFS fold transporters, the intracellular gates are typically formed from TM4 and TM5 packing against TM10 and TM11, and the extracellular gates are formed by TM1 and TM2 packing against TM7 and TM8 that are symmetry related pairs of transmembrane helices 52
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Handbook of ICU EEG Monitoring (2nd ed.)
Translation from animal research to human interpretation requires controlled validation across multiple study types
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