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A process using laboratory-scale 3 L bioreactors initially controlled at 37C during infection and 20 mL column GSH affinity chromatography columns was established previously, where the empty capsids present in cell culture harvests did not bind to the GSH ligand, and excellent full capsid purity was obtained in the GSH elution ( Prior to the GSH chromatography studies, the cell culture temperature setpoint during infection was studied to maximize the infectious titer
Homogeneous mix-incubate-measure type assay
Balmer ML, Ma EH, Bantug GR, Grhlert J, Pfister S, Glatter T, et al
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