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Afterwards, cells were washed with PBS, and incubated at room temperature for 30 min with an anti-rabbit IgG Alexa Fluor 546 antibody (1:100, #A-11035, Thermo Fisher Scientific, Waltham, MA, USA)

Identification of expression and function of the glucagon-like peptide-1 receptor in gastrointestinal smooth muscle

With regard to ALD, although alcohol-induced hepatotoxicity and oxidative stress are the key components contributing to its pathogenesis, recent studies have clearly shown that the immune response might also substantially contribute to the development of ALD, including its inflammatory component, alcoholic steatohepatitis (ASH) (Fig