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The samples were centrifuged, and the supernatant was subjected to a GSH assay based on a kinetic enzymatic recycling method that detects the oxidation of GSH by 5,5-dithiobis-2-nitrobenzoic acid (DTNB) and glutathione reductase to measure the GSH content in cells 74

Expected results Prior to the validation of our assay, we increased the expression of GSR by loading RAW264.7 Ms with oxidized low-density lipoprotein (oxLDL) and then stimulating with LPS

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