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However, this paradigm, in which immunotherapy works primarily by rebooting preexisting activated exhausted tumor-infiltrating CD8 + T cells [165], has been challenged by several recently published studies

Mild sonication may be used if needed

The off-window is part of the protocol continuous indefinite use is not supported by the component research

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The effects persisted beyond the peptide's pharmacokinetic half-life, supporting the "trigger" hypothesis (PMID 514474)