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After washing in PBS, primary antibodies (IgM-PE BioLegend 406507, IgD-APC BioLegend 405713, MARCO Santa Cruz sc-65353/ anti-rat PE Abcam ab97058 and Siglec-1 Biolegend 142417) diluted in PBS 10% FCS were incubated overnight at 4 C

Int J Mol Sci 25:5548 lter I, Akyl S, Demirel Z, Ko M, Conk-Dalay M, Kaymak-Ertekin F (2018) Optimization of phycocyanin extraction from Spirulina platensis using different techniques

Impatience and inconsistency Skipping doses, bouncing between strategies, or giving up early guarantees failure

A subgroup analysis by gender showed that there were no statistical differences in the percent change from baseline in visceral adipose tissue (VAT) and IGF-1 responses, respectively, between males and females

Impact of heavy metals on the environment and human health: novel therapeutic insights to counter the toxicity