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The researchers assessed the hepatic capacity for synthesizing urea-N (CUNS), which is a potential indicator of nitrogen metabolism in the liver

After three 15-min washes in Tris-buffered saline with Tween 20, membranes were incubated with an appropriate secondary antibody (#96232, Cell Signaling Technology, USA) conjugated to horseradish peroxidase diluted 1:1000 in blocking solution for 2 following three additional washes, protein reactivity was visualized using an ECL detection kit (Parstous, Iran)

It activates central amylin receptors to promote satiety, slow gastric emptying, and reduce food intake [2] [3]

Secondly, when homocysteine rises, it drives the MS reaction faster, which reduces 5mTHF

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