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However, they are also the most expensive and most time-consuming option
This consolidation is intended to reduce record fragmentation when protocols are transferred between operators or repeated after delays, and it helps preserve comparability across runs by anchoring documentation to a consistent reference
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The membranes were blocked at 37C for 1.5 h in 5% milk and then incubated 12-16 h at 4C with the following primary antibodies: COX2 (Abcam, Cat# ab179800), AKR1C1 (Abcam, Cat# ab192785), FTH1 (Abcam, Cat# ab75973), GPX4 (Abcam, Cat# ab125066) and -actin (Invitrogen, Cat# A1978)
The compounding nature of results means each day of proper protocol adherence builds on previous days
