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For the extraction, methanol and internal standards were added, followed by incubation for 15 min at 30C on a thermal shaker (650 rpm, TS1 ThermoShaker, Biometra, Gttingen, Germany), centrifugation (21,000 x g, 10 min, 4C, Heraeus Fresco 21 Centrifuge, Thermo Scientific, Schwerte, Germany), evaporation, and centrifugation with methanol again

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Targeting Obesity: GLP-1R/GIPR, GDF8 Leading the Way Rising Obesity Rates: A Global Threat Obesity, a chronic metabolic disease, has significantly impacted the health and quality of life for countless individuals

Ketogenic diets slow melanoma growth in vivo regardless of tumor genetics and metabolic plasticity