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All the other chemicals and reagents were bought from reputed manufacturers of analytical grade

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The math shifts: 50mg 2.5mg = 20 doses

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349 If the ulcers fail to show a 50% reduction after 4 weeks of appropriate management via conventional methods, several advanced therapies, including negative-pressure therapy, growth factor therapy, bioengineered tissue, acellular matrix tissue, stem cell therapy, hyperbaric oxygen therapy, and, most recently, topical oxygen therapy, can be considered