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Total RNA (20 g) prepared from the cultures of PAO1 and the gsh mutants were loaded into each lane

Blastocyst formation rate: Defined as the proportion of 2PN zygotes (not just of cleaved zygotes) which are at the blastocyst stage at Day 5 (116 2 h post-insemination) (18), We employed the Gardner system to evaluate the quality of blastocysts in terms of their expansion, inner cell mass, and trophoblast development (20)

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GLP-1 agonist drugs were initially developed and prescribed in the early 2000s to manage type 2 diabetes, and they can be beneficial for other health conditions under a physician's supervision (Darwish et al