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We subsequently performed quantitative measurement of ONSD using standardized amplitude modulation (A-scan) echography with tissue sensitivity settings, placing the 8 MHz A-scan probe on the temporal eye equator in primary gaze position, as previously described by others [24, 25]
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Repeated experiments demonstrated that the most reliable and robust differentiation was achieved using RPMI supplemented with B27 containing insulin, and supplemented with 3 M CHIR99021 (see Fig
