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Cells were lysed by sonication with 2 25 s pulses (0.9 s on and 0.1 s off)
These medications are not recommended in pregnancy
The gpoA mutant exhibited diminished survival across multiple organs relative to the wild-type strain, suggesting that GpoA contributes to pneumococcal pathogenesis
Population doubling was calculated as below: Determination of infection conditions for CRISPR-pooled screen The Brunello CRISPR knockout library contains an average of 4 guides per gene for a total of 77,441 sgRNAs and 1000 control guides
Together, these systems help protect retinal cells and support lens clarity, helping maintain healthy vision over time
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