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The following antibodies were used: anti-GAPDH (32233, Santa Cruz Biotechnology), rabbit anti-FLAG (F7425, Sigma-Aldrich/Merck) or M2 (F3165, Sigma-Aldrich/Merck), anti-LC3B (L7543, Sigma-Aldrich/Merck), anti-WIPI2 (purified mouse IgG clone 2A2 from the Tooze lab), anti-GFP (A11122, Thermo Fisher Scientific), anti-C16orf70 (ab181987, Abcam), anti-ATG16L1 (D6D5, Cell Signaling Technology), antiBeclin 1 (D40C5, Cell Signaling Technology), anti-ATG7 (D12B11 Cell Signaling Technology), anti-HA (C29F4, Cell Signaling Technology), and anti-BCAS3 (ab71162, Abcam)
however, evidence in people with type 2 diabetes remains limited
If you are on multiple medications, particularly insulin, sulfonylureas, or other glucose-lowering drugs, the interaction management requires more oversight than a text consultation typically provides
In rare cases, breathing difficulty or kidney strain
A tripartite transcription factor network regulates primordial germ cell specification in mice
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