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Key takeaways: The framework distinguishes between fat mass disease (mechanical effects) and sick fat disease (metabolic and inflammatory dysfunction)

Following fixation and staining as above, coverslips were mounted on slides with ProLong Gold antifade (Invitrogen) and imaged using a VisiTech iSIM for super-resolution imaging (100x or 150x oil immersion objectives, Olympus)

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In particular, the PI3K/AKT axis converts phosphatidylinositol-4,5-bisphosphate (PIP 2 ) to phosphatidylinositol 3,4,5-trisphosphate (PIP 3 )

This will likely delay niacin excretion with resultant higher niacin levels in the blood and for a longer duration