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Preparation The treatment area is cleansed and prepared to ensure comfort and safety

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The incubation was stopped after defined time points by precipitation of aliquots of enzymes with 2 volumes of cold internal standard solution (15 nM diphenhydramine in 10% methanol/acetonitrile)

The pro-inflammatory triggers apparently increased TNF- and IL-6 release, while GHK-Cu apparently reduced both cytokines

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