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After 10 min, the clotted blood samples were centrifuged for 5 min at 3000 rpm , and then serum was separated and transported into three new Eppendorf tubes until assay

IGF-1 DES: The deletion of the first three amino acids also reduces IGFBP binding, though the mechanism differs from LR3

Examination: Physical examination to identify the characteristic white patches on the skin

Other at-risk groups include people with coeliac or Crohns disease, those who have had weight loss surgery, and those with atrophic gastritis or pernicious anaemia

Some patients may experience weight regain if they do not continue with their healthy habits