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The MAM fraction was diluted 1:10 in mitochondrial resuspension buffer (250 mM mannitol, 5 mM HEPES, 0.5 M EGTA, pH 7.4), pelleted by ultracentrifugation at 100,000 g for 1 hour and prepared for western blot analysis as described above

Nat Plants 10(1):1324 Wang W, Pang M, Zhang Y et al (2025) Semaglutide mitigates ischemic brain injury by inhibiting ferroptosis via modulation of FoXO1 and DRP1 pathways

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