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Marchesini G, Bianchi G, Amodio P, Salerno F, Merli M, Panella C, et al

Two m slices of formalin-fixed paraffin-embedded (FFPE) tissue were dyed with hematoxillin/eosin (H&E) for determination of metabolic dysfunction-associated fatty liver disease (MASLD) and chromotrope-anilin blue (CAB) trichrome staining for fibrosis assessment

The mice were then decapitated and the entire brain was removed and immediately submerged in ice-cold, carbogen-saturated (95% O 2 and 5% CO 2 ) aCSF (126 mM NaCl, 2.8 mM KCl, 1.2 mM MgCl 2 , 2.5 mM CaCl 2 , 1.25 mM NaH 2 PO 4 , 26 mM NaHCO 3 and 5 mM glucose)

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