PC12 Adh cells was maintained in 90% DMEM/F12, 10% Fetal Bovine Serum, and 1%Penicillin-Streptomycin (5,000 U/mL) at 37 C in an Eppendorf Galaxy 170 S incubator (humidified, 37 C, 5% CO2)
As the study is based on data from earlier studies, ethical review and informed consent are not required
At low concentrations, RSV functions as an antioxidant by directly scavenging ROS and downregulation NADPH oxidases, thereby protecting against DNA damage and oxidative stress
Collaborative induction of inflammatory responses by dectin-1 and toll-like receptor 2
Minimize this risk by cleaning the rubber stopper with an alcohol swab before every draw, using a new sterile needle and syringe each time, not touching the needle to any surface before inserting it into the vial, and drawing the dose in a clean environment (not a dusty garage or outdoor setting)