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With a 10mg/mL concentration (1mL of water added), that 4mg dose requires drawing 0.4mL, which equals 40 units on a standard U-100 insulin syringe

This response is attributed to the release of pro-inflammatory mediators by immune cells, which sensitize nociceptors and subsequently induce pain (46)

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Spray format is used in research applications where a pre-mixed solution is preferred