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Jin (2008)
Enzyme expression was induced by the addition of 100 M isopropyl -d-1-thiogalactopyranoside, the cultures were incubated for another 3.5 h at 30 C, 140 rpm using a 5 cm shaking diameter, and the final OD 600 (optical density at 600 nm) was determined using a CO8000 cell density meter (WPA biowave)
As described above, TGF--induced HK-2 cells are accompanied by ferroptosis activation, and HK-2 cells treated with TGF- also presented increased protein and mRNA levels of fibronectin, collagen-1, and -SMA
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