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The analytical data was found to be in agreement with the previous literature 46

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The researchers evaluated various oxidative stress markers, including protein carbonylation, protein-bound 4-hydroxy-2-nonenal (HNE), levels of total and oxidized glutathione (GSH), concentrations of heat shock protein (HSP) and thioredoxin (TRX), in amniotic fluid samples

Aqueous working solutions should be prepared immediately prior to experimental administration and maintained at 4C for no longer than 24 to 48 hours

10.1007/s00429-016-1218-9 12 FinucaneD