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The GSH levels of the BSO-treated cells quantified using our RT-based flow cytometry method is well correlated to the values measured using cell lysates with a glutathione reductase-based biochemical assay ( R 2 =0.99, Fig
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Two days after siRNA transfection, cells were transfected with an additional 0.5 l PDS5B siRNA (jkSIR012: 20 M), 1.2 g HA-TwinStrepTagII-PDS5B wild type or the 17 A mutant, 0.8 g I-SceI expression vector (pCBASce), and 5 l Lipofectamine 2000
This literature also points out that glutathione is a key molecule in preventing UCB-induced cell death, which may help to investigate the use of bilirubin in cancer control
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