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10.1093/ndt/gfad250 105 MakhroA.HnggiP.GoedeJ
The off period allows assessment of sustained metabolic benefits after discontinuation
The supervision piece matters more than it sounds
For the generation of triple mutants, the kanamycin cassette was removed from the gshA, gsiC or gshA, gsiB double deletion mutant using the plasmid 709-FLPe as indicated by the supplier (Gene Bridges, Heidelberg, Germany)
There may also be a connection to sex hormones because most lupus patients are women of reproductive age with high levels of estrogen and low levels of progesterone, a hormone that supports pregnancy (x)
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