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Using a sterile scalpel and forceps, the bottoms of the cell strainers were removed and placed in new, autoclaved vials with germ-free fly food

Prolactin and the dietary protein/carbohydrate ratio regulate the expression of snat2 amino acid transporter in the mammary gland during lactation

Allow vials to reach room temperature for 15 to 30 minutes before opening to prevent condensation Disinfect rubber stoppers with alcohol swab and allow complete evaporation Draw the calculated volume of bacteriostatic water into syringe Angle needle against vial wall and slowly inject water down the side, allowing it to gently slide onto powder Never squirt water directly onto lyophilized powder as this damages peptide structure Gently swirl or roll vial between palms to dissolve

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For further evaluation of this model, we generated the receiver operating characteristic (ROC) curves in R timeROC (v0.4)