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In patients with T2DM and hepatic steatosis, Cuthbertson et al
Bomfim TR, Forny-Germano L, Sathler LB, et al
Embodiment 3 [0107] On the basis of Example 1, the condensing agent used when coupling Boc-His(Trt)-Aib-OH was replaced by HATU / DIEA, and the obtained semaglutide crude peptide HPLC detection results were as follows Image 6 As shown, the purity of semaglutide is 71.369%, and the maximum unmixed value is 4.609%
Protein precipitation especially prior to flourimetric analysis is the method of choice for extraction in several reports [15, 16] due to its simplicity and greenness as it requires small amount of organic solvent to precipitate the proteins followed by a simple step of centrifugation and filtration as previously discussed under For spiked plasma extraction and analysis in Methods section
A., Dunn, J., Haupt, A., Milicevic, Z., & Coskun, T
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