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All samples from the topical steroids-only group passed quality control and were confirmed to have sufficient DNA concentration for downstream analysis
Then, 10 l of LacAnc100 or LaccID was added to flat-bottom 96-well plates (Greiner Bio-One, 655101) and 190 l of reaction mixtures containing PBS and different substrates were added with the help of a Multidrop system (Multidrop Combi, Thermo Scientific)
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Anionic liposomes have superior solution stability, reduced aggregation in comparison to neutrally charged liposomes, and enhanced endocytosis compared to neutral and cationic liposomes [22, 23]
Optional: GHK-Cu nasal spray 200 mcg twice daily as a systemic supplement
