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Hair mesotherapy is generally not considered very painful, but the level of discomfort can vary depending on individual sensitivity, the needle depth used and the length of the session

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This distinction is also reflected in the methodology employed to quantify synaptic density, predominantly carried out in post-mortem tissue (e.g., EM and IHC), whereas synaptic function is by default linked to experiments in living tissues, using electrophysiology, electroencephalography, or brain imaging with [ 18 F]FDG PET and fMRI

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Slack RJ, Macdonald SJF, Roper JA, Jenkins RG, Hatley RJD