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The extracts were vortexed and centrifuged at 20,000 rcf and 4C for 10 min, and the supernatant was mixed with TBARS reagent (0.5% thiobarbituric acid in 20% trichloroacetic acid) at a 1:2 ratio

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For each obtained lipidome (ER, mitochondria, whole cell), mol percent were calculated and used for final evaluation

[19] [20] Improved wellbeing: Patients with confirmed deficiency often report enhanced energy and cognitive function following appropriate replacement therapy