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coli BL21 (DE3) (Sigma-Aldritch) at 110-fold coverage of the library size, and following 1-hr recovery from transformation, cells were immediately diluted into LB media (Gibco) containing kananycin (selecting for presence of the library) and 10 uM IPTG (inducer for library expression), beginning the massively parallel inhibition measurements
Finally, the sulfation rate also increased the number of zonal necrosed cells, with the variation in the number of necrosed cells increasing from 0.9 times at 20% zonation to 1.8 times at 80% zonation
