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As a negative () control experiments, HpGroEL was omitted from the reaction mixture

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Patients should be educated about red flag symptoms requiring immediate medical attention, including severe persistent abdominal pain (especially if radiating to the back, suggesting possible pancreatitis), right upper quadrant pain with fever or jaundice (potential gallbladder disease), inability to keep fluids down for more than 24 hours, syncope, chest pain, or difficulty breathing