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2.4 Assessment of mitochondrial function and cell viability Mitochondrial function was assessed by measuring adenosine triphosphate (ATP) content and mitochondrial membrane potential using ATP Assay Kit-Luminescence and JC-1 MitoMP Detection Kit, respectively, following the manufacturers instructions (Dojindo Laboratories, Mashiki, Japan)
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This can lead to: Increased appetite Difficulty burning stored fat Fatigue Weight gain around the abdomen Increased risk of Type 2 diabetes Hormonal Changes Age, menopause, stress, poor sleep, thyroid disorders, and certain medications can all disrupt hormones that regulate hunger and fullness
On the other hand, treatments with exogenous SA resulted in increasing cellular GSH levels and elevated activities of glutathione reductase and glutathione S-transferase enzymes, along with enhanced virus resistance (Srivastava and Dwivedi, 1998
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