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Treatment with CDDO-Me increased NRF2 expression but not to the level observed in the liver of Gclc L-KO mice, as we could only detect substantial NRF2 expression after enriching the whole protein lysate for a nuclear fraction (Fig

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For intracellular staining of hexokinase-1, cells were stained for extracellular markers, fixed in 4% FA at RT, and stained with Anti-Hexokinase 1 (Abcam ab184818) diluted 1:100 in PBS 0.1% Tween-20 at RT