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The free peptide reconstitution calculator makes this math simple

The eluted proteins were confirmed by running on a 12% SDS Gel, and the proteins were pooled together and subjected to overnight dialysis (50 mM Tris pH 7.5, 125 mM NaCl, 10% glycerol and 1 mM DTT) at 4 C

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They, therefore, suggested administering a second dose of PRP where necessary at about three months in the case of partial recovery as a boost dose [23]