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While ionic copper can promote oxidative damage through Fenton reactions, GHK-Cu is studied as a protected copper delivery systemthe peptide binds copper tightly, preventing redox cycling while delivering it to cells in a bioavailable form
PMC9664149
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The next day, the cells were treated with the test compounds for the indicated times and then incubated with C11-BODIPY 581/591 (Thermo Fisher, catalog D3861) at a working concentration of 5 M for 30 min at 37 C
(PubMed) Smith AR, Hagen TM
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