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Ultra-filtration (to wash out un-reacted free trisulfides) Trisulfide-pretreated HGF solutions were loaded into Amicon ultra-filtration devices (with a 10-kDa MWCO polyethersulfone (PES)-membrane) and spun at the recommended centrifugal force (typically 6000-7000 rpm) for 1030 min at 4 or until the desired volume was reached
While our study highlights the significance of hepsin in the context of liver protection and its potential therapeutic implications in APAP-induced hepatotoxicity, there are limitations to consider when evaluating hepsin as a candidate drug or therapeutic strategy
This article does not contain any studies with human participants or animals performed by any of the authors
Most documented protocols treat glutathione supplementation as a maintenance intervention rather than a cycled compound
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