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Manders overlap coefficient was primarily used, defined as i( R i G i )i R i 2 i G i 2 where R i is the intensity of the first fluorophore in an individual pixel and G i is the corresponding intensity for the second fluorophore in the same pixel

IM's shorter duration (34 hours) would create significant trough periods between doses, requiring either more frequent injections or accepting subtherapeutic windows

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