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doi: 10.2337/diacare.21.9.1414 4 WhitingDRGuariguataLWeilCShawJ
An LPS-free peptide indicates that the MT-1 has been processed to minimize lipopolysaccharide contamination, which is critical for laboratory experiments where endotoxins could interfere with cellular signaling, receptor assays, or biochemical readouts
Consistently, regardless of the initial positions of palmitate either located in the membrane as seen in the cryo-EM structure or placed outside of the membrane, our MD simulations found that the -carboxylate spacer stably inserted into the TM1-TM2 cleft and the 16-carbon palmitic chain is deeply buried in the receptor-lipid interface, contributing multiple strong contacts to stabilize the complexes (Supplementary Fig
Due to the complementary research interest of these peptides, this blend remains a compelling subject for ongoing laboratory investigation
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