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In addition, a MAGPIX Multiplex Reader was used to measure 25 inflammatory mediators simultaneously in plasma from female [Young ( n = 8), Old Con ( n = 15), and Old mAb ( n = 16)] and male mice [Young ( n = 8), Old Con ( n = 14), and Old mAb ( n = 16)], including: G-CSF, GM-CSF, IFN-, IL-1, IL-1, IL-2, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17, CXCL-10, CXCL-1, MCP-1, MIP-1, MIP-1, MIP-2, RANTES, and TNF- (MCYTOMAG-70K-PMX

Armogida et al

Although a precise understanding of the underlying mechanism depends on detailed structural analyses, our results suggest that the K29 linkage is not only resistant by itself to OTUD5, but also modestly stabilizes the mixed/branched K48 linkage