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[ 3 H] GSSG uptake assay in immunopurified ER SLC33A1 knockout HEK293T or Slc33a1 knockout KPK cells expressing EMC3-mScarlet-3xHA (ER-tag) complemented with a vector control or SLC33A1 cDNA were treated with 1 mM BSO for 48 hours prior for ER isolation