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Single-cell gel electrophoresis (comet assay) Using an image analysis system (Casp lab 1.2.3 b2) attached to a fluorescent microscope (Nikon Eclipse E200, Nikon) with proper filters, 250 cells per concentration, with 50 cells per slide, were randomly scored
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Full results were presented at the American Diabetes Associations 85th Scientific Sessions alongside the REDEFINE 1 data
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