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Emous, M., Wolffenbuttel, B

After confirming that the concentration of NADPH in our lysates was within the linear detection range of the assay (Fig 3A), we treated LPS-stimulated (6 h) RAW264.7 Ms with 2-DG and diamide and quantified NADPH kinetics by obtaining lysates at 5-minute intervals for a total of 25 minutes (Fig 3B)

However, while promising evidence in experimental animals demonstrate that it is possible to impair BAT thermogenesis (i.e., by beta-adrenergic blockade), no intervention has so far been able to increase it (Tupone et al., 2014)

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