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Subcutaneous injection into abdominal fat or near the site of injury (as commonly practiced by self-experimenters) is the most reported approach, but this is not a clinical protocol

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Record the date of first vial entry and discard after 28 days regardless of remaining volume and the bacteriostatic preservation system provides a defined multi-use window, not indefinite sterility maintenance

But product quality is not always the issue

The most common method, also introduced by Schilling, is to inject a high dose of unlabeled B12 to saturate all binding capacities of the B12 binding proteins, and thereby facilitating urinary excretion of the absorbed radioactively labeled B12