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Cells were then washed, lysed in a 0.5 M Tris-HCl, pH 7.4 buffer containing 0.5% Nonidet P-40, 20 mM EDTA, 10 mM NaF, 2 mM benzamidine and a protease inhibitor cocktail (Roche), and cleared by centrifugation
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Metformin Metformin is an antihyperglycemic agent which improves glucose tolerance in patients with type 2 diabetes mellitus, lowering both basal and postprandial plasma glucose
Beutler LR, Chen Y, Ahn JS, Lin YC, Essner RA, Knight ZA
Start lower, then build up if your body handles it well
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